United States Patent: 6881832
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چکیده
Mammalian signal peptides can be used to aid in the secretion of mammalian proteins that are normally not secreted. By inactivating signals normally present in the 3' UTRs of mRNAs encoding proteins that are normally not secreted from mammalian cells, the proportion of such mRNA molecules directed to free and cytoskeletal bound polysomes can be reduced. Inventors: Hesketh; John Edward (Newcastle, GB), Tauler; Albert (Barcelona, ES), Pryme; Ian Fraser (Bergen, NO) Assignee: UNI Targeting Research AS (Bergen, NO) Appl. No.: 09/518,190 Filed: March 2, 2000 Foreign Application Priority Data Sep 05, 1997 [GB] 9718908 Current U.S. Class: 536/23.5 ; 435/252.3; 435/320.1; 435/471; 435/69.1 Current International Class: C12P 21/06 (20060101); C12N 1/20 (20060101); C12N 15/00 (20060101); C12N 15/74 (20060101); C07H 21/04 (20060101); C07H 21/00 (20060101) http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO...1=AND&d=ptxt&s1=6,881,832&OS=6,881,832&RS=6,881,832 (1 av 16)30/11/2006 17:04:15 United States Patent: 6881832 Field of Search: 536/23.5 530/402 435/69.1,70.1,71.1,71.2,252.3,320.1,471 Foreign Patent Documents 0 226 057 May., 1988 EP 0 266 057 May., 1988 EP 0 279 582 Aug., 1988 EP 0 279 582 Aug., 1988 EP WO 91/13151 Sep., 1991 WO WO 93/04165 Mar., 1993 WO
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